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KMID : 1007520020110010089
Food Science and Biotechnology
2002 Volume.11 No. 1 p.89 ~ p.92
Antimicrobial Effect of Bifidobacterium breve and Bifidobacterium infantis against Salmonella Typhimurium KCTC 1925 and E. coli O157:H7 ATCC 43895
Kim, Jeong Weon
Lee, So Jin/Park, Kwan Hwa
Abstract
The antimicrobial effect of two species of bifidobacteria against Salmonella Typhimurium (KCTC 1925) and E. coli O157:H7 (ATCC 43895) was examined for the presence of additional mechanisms of inhibition other than acidity. Bifidobacterium breve (ATCC 15700) and B. infantis (ATCC 25962) were cultured anaerobically for two days at 37¡É, and the cells were removed to obtain the spent broths, of which the pH was adjusted to 7.0. When 20-fold concentrated spent broth by bifidobacteria was applied to the lawns of S. Typhimurium and E. coli O157:H7, inhibitory zones appeared clearly, whereas the control spent broth used by E. coli did not. When S. Typhimurium and E. coli O157:H7 were cultivated in the mixture of fresh and bifidobacteria-spent broths (1:1) with an adjusted pH of 7.0, the cell counts were lower than the control by 2.4 logs for S. Typhimurium and by 1.6 logs for E. coli O157:H7. The above results suggest that B. breve and B. infantis have inhibition activities other than acid production against S. Typhimurium and E. coli )157:H7.
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